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dc.contributor.authorPaniagua Correas, Candelas
dc.contributor.authorKirby, Andrew R.
dc.contributor.authorGunning, A. Patrick
dc.contributor.authorMorris, Victor J.
dc.contributor.authorMatas-Arroyo, Antonio Javier 
dc.contributor.authorQuesada-Felice, Miguel Ángel 
dc.contributor.authorMercado-Carmona, José Ángel 
dc.date.accessioned2015-06-26T10:58:19Z
dc.date.available2015-06-26T10:58:19Z
dc.date.created2015
dc.date.issued2015-06-26
dc.identifier.urihttp://hdl.handle.net/10630/9964
dc.description.abstractRapid loss of firmness occurs during strawberry (Fragaria × ananassa Duch) ripening, resulting in a short shelf life and high economic losses. The disassembly of cell walls is considered the main responsible for fruit softening, being pectins extensively modified during strawberry ripening (Paniagua et al. 2014). Atomic force microscopy allows the analysis of individual polymer chains at nanostructural level with a minimal sample preparation (Morris et al., 2001). The main objective of this research was to compare pectins of green and red ripe strawberry fruits at the nanostructural level to shed light on structural changes that could be related to softening. Cell walls from strawberry fruits were extracted and fractionated with different solvents to obtain fractions enriched in a specific component. The yield of cell wall material, as well as the amount of the different fractions, decreased in ripe fruits. CDTA and Na2CO3 fractions underwent the largest decrements, being these fractions enriched in pectins supposedly located in the middle lamella and primary cell wall, respectively. Uronic acid content also decreased significantly during ripening in both pectin fractions, but the amount of soluble pectins, those extracted with phenol:acetic acid:water (PAW) and water increased in ripe fruits. Monosaccharide composition in CDTA and Na2CO3 fractions was determined by gas chromatography. In both pectin fractions, the amount of Ara and Gal, the two most abundant carbohydrates, decreased in ripe fruits. The nanostructural characteristics of CDTA and Na2CO3 pectins were analyzed by AFM. Isolated pectic chains present in the CDTA fraction were significantly longer and more branched in samples from green fruits than those present in samples obtained from red fruit. In spite of slight differences in length distributions, Na2CO3 samples from unripe fruits displayed some longer chains at low frequency that were not detected in ripe fruits. Pectin aggregates were more frequently observed in green fruit samples from both fractions. These results support that pectic chain length and the nanostructural complexity of the pectins present in CDTA and Na2CO3 fractions diminish during strawberry fruit development, and these changes, jointly with the loss of neutral sugars, could contribute to the solubilization of pectins and fruit softening. Paniagua et al. (2014). Ann Bot, 114: 1375-1383 Morris et al. (2001). Food Sci Tech 34: 3-10 This research was supported by FEDER EU Funds and the Ministerio de Educación y Ciencia of Spain (grant reference AGL2011-24814)es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectFresas - Fisiologíaes_ES
dc.subject.otherStrawberryes_ES
dc.subject.otherFragariaes_ES
dc.subject.otherFruit softeninges_ES
dc.subject.otherPectinses_ES
dc.subject.otherAFMes_ES
dc.subject.otherFruit ripeninges_ES
dc.subject.otherCell walles_ES
dc.titleNanostructural changes in cell wall pectins during strawberry fruit ripening assessed by atomic force microscopyes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXIV Congreso Hispano-Luso de Fisiología Vegetales_ES
dc.relation.eventplaceToledoes_ES
dc.relation.eventdate14/06/2015es_ES
dc.rights.ccby-nc-nd


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