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Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform.
dc.contributor.author | Labella Vera, Alejandro Manuel | |
dc.contributor.author | Gémez Mata, Juan | |
dc.contributor.author | Vera, Isabel | |
dc.contributor.author | Bandin, Isabel | |
dc.contributor.author | Leiva Rebollo, Rocío | |
dc.contributor.author | Borrego-García, Juan José | |
dc.contributor.author | García-Rosado, Esther | |
dc.date.accessioned | 2024-10-10T08:42:05Z | |
dc.date.available | 2024-10-10T08:42:05Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | A. Labella, J. Gémez, I. Vera, I. Bandín, R. Leiva, J.J. Borrego, E. Garcia-Rosado, Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform, Fish & Shellfish Immunology, Volume 91, 2019, Pages 391-392, ISSN 1050-4648, https://doi.org/10.1016/j.fsi.2019.04.077. (https://www.sciencedirect.com/science/article/pii/S1050464819303158) | es_ES |
dc.identifier.uri | https://hdl.handle.net/10630/34600 | |
dc.description.abstract | Nervous necrosis virus (NNV) is the causative agent of the viral encephalopathy and retinopathy, a disease that affects cultured Senegalese sole (Solea senegalensis). A NNV reassortant (Ss160.03), combining genomic segments from red-spotted grouper nervous necrosis virus (RGNNV) and striped jack nervous necrosis virus (SJNNV) genotypes, has been previously isolated from Senegalese sole, being highly virulent to this fish species. The RNA-Seq technology has been used in a previous study to comparatively analyse Senegalese sole transcriptomes in two organs (head kidney and eye/brain) after infection with two NNV virus with different levels of virulence to that fish species, a highly virulent reassortant isolate (wSs160.03) and a less virulent mutant reassortant obtained by reverse genetics (rSs160.03247þ270). To validate previous RNA-Seq results, a 112- assay OpenArray® platform (Thermofisher) has been designed. This platform included 89 genes chosen according to transcriptomic changes observed by RNA-Seq (covering PRRs, type I IFN response, signal transduction, inflammation, virus responsive genes, and apoptosis), 17 genes selected based on their previously described relation with the immune response against fish viral infections, and 6 control genes (including 3 endogenous genes and 3 viral genes). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Peces - Agentes patógenos | es_ES |
dc.subject.other | Solea senegalensis | es_ES |
dc.subject.other | Reassortant nervous necrosis virus | es_ES |
dc.subject.other | Differentially expressed genes | es_ES |
dc.subject.other | Immune response | es_ES |
dc.subject.other | Open-array | es_ES |
dc.title | Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform. | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.centro | Facultad de Ciencias | es_ES |
dc.relation.eventtitle | International Conference on Fish and Shellfish Immunology | es_ES |
dc.relation.eventplace | Las Palmas de Gran Canaria, España | es_ES |
dc.relation.eventdate | 16/06/2019 | es_ES |
dc.identifier.doi | 10.1016/j.fsi.2019.04.077 | |
dc.rights.cc | Atribución 4.0 Internacional | * |