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dc.contributor.authorPérez-Cano, Ana María
dc.contributor.authorPérez-Berlanga, José Manuel
dc.contributor.authorÁvila-Gámiz, Fabiola
dc.contributor.authorSantín-Núñez, Luis Javier 
dc.contributor.authorLadrón de Guevara-Miranda, David
dc.date.accessioned2024-07-09T11:17:07Z
dc.date.available2024-07-09T11:17:07Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/10630/32009
dc.description.abstractMechanisms underlying relapse into cocaine are not yet fully understood. Animal studies suggest a pivotal involvement of the infralimbic (IL) division of the medial prefrontal cortex, but causal evidence is lacking. Here, we employed chemogenetics to selectively modulate the IL during reinstatement of cocaine-related behaviours in the conditioned place preference (CPP) model. To this aim, C57BL/6J mice (N = 28) received intra-IL microinjections of adenoassociated viral vectors containing either silencing (AAV5-CaMKIIα-hM4Di-mCherry) or stimulatory (AAV5-CaMKIIα-hM3Dq-mCherry) designer receptors, or a control vector (AAV5-CaMKIIα-mCherry). Animals were trained to acquire a CPP induced by increasing doses of cocaine (2-16 mg/kg) and later submitted to forced extinction. Four weeks after AAV infusion, mice received Clozapine N-oxide (5 mg/kg) 30 minutes before undergoing a cocaine-primed (7.5 mg/kg) CPP reinstatement test. Ninety minutes later animals were perfused, and brains dissected to analyse the expression of mCherry and c-Fos proteins in the IL. We found that CPP reinstatement was significantly increased after silencing IL compared to the control group, while blocked after selective stimulation of this brain region. In turn, immunofluorescence analyses revealed a ~0.5-fold decrease and a ~3-fold increase in mCherry /c-Fos co-labelling in AAV-hM4Di and AAV-hM3Dq groups (respectively) compared to the control group. Taken together, our data indicate that the IL is causally involved in the reinstatement of cocaine-related maladaptive behaviours and stands out as a target for modulation to prevent cocaine relapse.es_ES
dc.description.sponsorshipFunding: PID2020-113806RB-I00, B4-2023-16, FPU20/00908, PREDOC_01094, POSTDOC_ 21_00222. II Plan Propio de Investigación, Transferencia y Divulgación Científica de la Universidad de Málaga. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectCocaína - Modelos animaleses_ES
dc.subjectCocaína - Efectos fisiológicoses_ES
dc.subject.otherCocainees_ES
dc.subject.otherDREADDes_ES
dc.subject.otherExtinctiones_ES
dc.subject.otherInfralimbic cortexes_ES
dc.subject.otherReinstatementes_ES
dc.titleSelective modulation of the infralimbic cortex activity regulates reinstatement of cocaine-context associations in mice.es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Psicología y Logopediaes_ES
dc.relation.eventtitleFENS Forum 2024es_ES
dc.relation.eventplaceViena, Austriaes_ES
dc.relation.eventdate06/2024es_ES


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