The recent advent of long read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford
Nanopore technology (ONT), have led to substantial improvements in accuracy and computational cost in
sequencing genomes. However, de novo whole-genome assembly still presents significant challenges related to
the quality of the results. Pursuing de novo whole-genome assembly remains a formidable challenge, underscored
by intricate considerations surrounding computational demands and result quality. As sequencing accuracy and
throughput steadily advance, a continuous stream of innovative assembly tools floods the field. Navigating this
dynamic landscape necessitates a reasonable choice of sequencing platform, depth, and assembly tools to
orchestrate high-quality genome reconstructions. This comprehensive review delves into the intricate interplay
between cutting-edge long read sequencing technologies, assembly methodologies, and the ever-evolving field of
genomics. With a focus on addressing the pivotal challenges and harnessing the opportunities presented by these
advancements, we provide an in-depth exploration of the crucial factors influencing the selection of optimal
strategies for achieving robust and insightful genome assemblies.
