Bicuspid aortic valve (BAV) is the most common human congenital cardiac malformation. Although the etiology is unknown for
most patients, formation of the 2 main BAV anatomic types (A and B) has been shown to rely on distinct morphogenetic mechanisms. Animal models of BAV include 2 spontaneous hamster strains and 27 genetically modified mouse strains. To assess
the value of these models for extrapolation to humans, we examined the aortic valve anatomy of 4340 hamsters and 1823 mice
from 8 and 7 unmodified strains, respectively. In addition, we reviewed the literature describing BAV in nonhuman mammals. The
incidences of BAV types A and B were 2.3% and 0.03% in control hamsters and 0% and 0.3% in control mice, respectively.
Hamsters from the spontaneous model had BAV type A only, whereas mice from 2 of 27 genetically modified strains had BAV type
A, 23 of 27 had BAV type B, and 2 of 27 had both BAV types. In both species, BAV incidence was dependent on genetic background. Unlike mice, hamsters had a wide spectrum of aortic valve morphologies. We showed interspecific differences in the
occurrence of BAV between humans, hamsters, and mice that should be considered when studying aortic valve disease using
animal models. Our results suggest that genetic modifiers play a significant role in both the morphology and incidence of BAV. We
propose that mutations causing anomalies in specific cardiac morphogenetic processes or cell lineages may lead to BAV types A, B, or both, depending on additional genetic, environmental, and epigenetic factors
