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dc.contributor.authorOrozco-Navarrete, Begoña
dc.contributor.authorKaczmarska, Zuzanna
dc.contributor.authorDupeux, Florine
dc.contributor.authorGarrido-Arandia, María
dc.contributor.authorPott, Delphine
dc.contributor.authorDíaz-Perales, Araceli
dc.contributor.authorCasañal, Ana
dc.contributor.authorMárquez, José A.
dc.contributor.authorValpuesta-Fernández, Victoriano 
dc.contributor.authorMerchante-Berg, Catharina
dc.date.accessioned2024-02-01T12:13:57Z
dc.date.available2024-02-01T12:13:57Z
dc.date.created2024
dc.date.issued2019-11-27
dc.identifier.citationOrozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Arandia M, Pott D, Perales AD, Casañal A, Márquez JA, Valpuesta V, Merchante C. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. J Agric Food Chem. 2020 Sep 30;68(39):10951-10961. doi: 10.1021/acs.jafc.9b05714. Epub 2020 Jan 3. PMID: 31774998; PMCID: PMC7644122.es_ES
dc.identifier.urihttps://hdl.handle.net/10630/29613
dc.description.abstractAlthough strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient’s previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.es_ES
dc.description.sponsorshipThis work was supported by the following grants: a BIO2013-44199-R (MEC, Spain) to V.V., the Ramón y Cajal program RYC-2017-22323 (MINECO, Universidad de Málaga, Spain) to C.M., a BES-2014-068723 predoctoral fellowship to B.O.-N., a European Community’s Seventh Framework Programme H2020 under iNEXT (H2020 Grant #653706) to J.A.M., and a BIO2017-84548-R (MINECO, Spain) and Instituto de Salud Carlos III (ISCIII) cofounded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/006/003) to A.D.P.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFresases_ES
dc.subjectAlergenoses_ES
dc.subject.otherFra a 1es_ES
dc.subject.otherFragaria x ananassaes_ES
dc.subject.otherAllergenes_ES
dc.subject.otherPR-10es_ES
dc.subject.otherIgE bindinges_ES
dc.subject.other3D-structurees_ES
dc.subject.otherHypoallergenic mutantses_ES
dc.titleStructural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doi10.1021/acs.jafc.9b05714
dc.rights.ccAtribución 4.0 Internacional
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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