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dc.contributor.authorRamiro-Gutiérrez, María Lourdes
dc.contributor.authorSantos-Ruiz, Leonor 
dc.contributor.authorBorrego-González, Sara
dc.contributor.authorBecerra-Ratia, José 
dc.contributor.authorDíaz-Cuenca, Aránzazu
dc.date.accessioned2024-02-01T07:30:57Z
dc.date.available2024-02-01T07:30:57Z
dc.date.issued2016-07-15
dc.identifier.citationRamiro Gutiérrez, M.L., Santos Ruiz, L., Borrego González, S., Becerra, J., & Díaz Cuenca, A. (2016). In vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2CaOP2O5 scaffold. Microporous and Mesoporous Materials, 229, 31-43. https://doi.org/10.1016/j.micromeso.2016.04.018es_ES
dc.identifier.urihttps://hdl.handle.net/10630/29550
dc.description.abstractA hierarchical scaffold, SP1_h_HA, consisting of a biomimetic nano-hydroxyapatite surface coating growth onto a reticulated structure having a nano-organized porous texture was fabricated and functionally studied in vitro using osteoprogenitor cells. Three scaffold materials (designated as SP0-l, SP0-h and SP1-h) were also prepared through modifications of the processing variables as control materials. The scaffolds were characterized showing well-interconnected micron-sized voids and a nano (4-6 nm)-organized porosity. In order to evaluate potential local risks and performance over mammalian cells the scaffolds were studied in comparison with a commercial clinical grade scaffold material, ProOsteon® 500R. MC3T3-E1 pre-osteoblast viability was evaluated using the resazurin assay and field emission gun scanning electron microscopy (FEG-SEM), showing in all cases good proliferative response. Alkaline phosphatase (ALP) production and analysis of the differentiation marker osteocalcin (OC), both in non-osteoinductive and osteoinductive media, were assessed using colorimetric and RT-PCR methods. The implementation of the new scaffold processing variables enhanced ALP activity with respect to the SP0-l control material. The cell proliferation, ALP activity, and mRNA OC expression response to SP1_h_HA scaffold were higher than those observed after the use of ProOsteon® 500R. In addition, SP1_h_HA scaffold showed a two stage sustained release of gentamicin sulfate (GS) instead of the quick release shown by ProOsteon® 500R. These results suggest that our synthesized scaffold could be effective for antibiotic delivery and bone regeneration and a better option than ProOsteon® 500Res_ES
dc.description.sponsorshipMICINN (BIO2009-13903-C02-02) Consejería de Economía, Ciencia e Innovación de la Junta de Andalucía (P10-CTS-06681) ISCiii RETICS, Red Española de Terapia Celular (TerCel) (RD12/0019/0032)es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.subjectRegulación celulares_ES
dc.subjectCélulas - Diferenciaciónes_ES
dc.subjectNanotecnologíaes_ES
dc.subjectTejidos (Biología) - Cultivoes_ES
dc.subject.otherMesoporous bioactive glasses_ES
dc.subject.otherSBA-15es_ES
dc.subject.otherProOsteon (R)es_ES
dc.subject.otherOsteoprogenitor cells differentiationes_ES
dc.subject.otherGentamicines_ES
dc.subject.otherSOL-GEL GLASSESes_ES
dc.subject.otherBONE REGENERATIONes_ES
dc.subject.otherOSTEOBLASTIC DIFFERENTIATIONes_ES
dc.subject.otherDrug releasees_ES
dc.subject.otherHYDROXYAPATITE MODELes_ES
dc.subject.otherOSTEOMYELITISes_ES
dc.subject.otherBIOMATERIALSes_ES
dc.titleIn vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2-CaO-P2O5 scaffoldes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doi10.1016/j.micromeso.2016.04.018
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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