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In vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2-CaO-P2O5 scaffold
dc.contributor.author | Ramiro-Gutiérrez, María Lourdes | |
dc.contributor.author | Santos-Ruiz, Leonor | |
dc.contributor.author | Borrego-González, Sara | |
dc.contributor.author | Becerra-Ratia, José | |
dc.contributor.author | Díaz-Cuenca, Aránzazu | |
dc.date.accessioned | 2024-02-01T07:30:57Z | |
dc.date.available | 2024-02-01T07:30:57Z | |
dc.date.issued | 2016-07-15 | |
dc.identifier.citation | Ramiro Gutiérrez, M.L., Santos Ruiz, L., Borrego González, S., Becerra, J., & Díaz Cuenca, A. (2016). In vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2CaOP2O5 scaffold. Microporous and Mesoporous Materials, 229, 31-43. https://doi.org/10.1016/j.micromeso.2016.04.018 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10630/29550 | |
dc.description.abstract | A hierarchical scaffold, SP1_h_HA, consisting of a biomimetic nano-hydroxyapatite surface coating growth onto a reticulated structure having a nano-organized porous texture was fabricated and functionally studied in vitro using osteoprogenitor cells. Three scaffold materials (designated as SP0-l, SP0-h and SP1-h) were also prepared through modifications of the processing variables as control materials. The scaffolds were characterized showing well-interconnected micron-sized voids and a nano (4-6 nm)-organized porosity. In order to evaluate potential local risks and performance over mammalian cells the scaffolds were studied in comparison with a commercial clinical grade scaffold material, ProOsteon® 500R. MC3T3-E1 pre-osteoblast viability was evaluated using the resazurin assay and field emission gun scanning electron microscopy (FEG-SEM), showing in all cases good proliferative response. Alkaline phosphatase (ALP) production and analysis of the differentiation marker osteocalcin (OC), both in non-osteoinductive and osteoinductive media, were assessed using colorimetric and RT-PCR methods. The implementation of the new scaffold processing variables enhanced ALP activity with respect to the SP0-l control material. The cell proliferation, ALP activity, and mRNA OC expression response to SP1_h_HA scaffold were higher than those observed after the use of ProOsteon® 500R. In addition, SP1_h_HA scaffold showed a two stage sustained release of gentamicin sulfate (GS) instead of the quick release shown by ProOsteon® 500R. These results suggest that our synthesized scaffold could be effective for antibiotic delivery and bone regeneration and a better option than ProOsteon® 500R | es_ES |
dc.description.sponsorship | MICINN (BIO2009-13903-C02-02) Consejería de Economía, Ciencia e Innovación de la Junta de Andalucía (P10-CTS-06681) ISCiii RETICS, Red Española de Terapia Celular (TerCel) (RD12/0019/0032) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | es_ES |
dc.subject | Regulación celular | es_ES |
dc.subject | Células - Diferenciación | es_ES |
dc.subject | Nanotecnología | es_ES |
dc.subject | Tejidos (Biología) - Cultivo | es_ES |
dc.subject.other | Mesoporous bioactive glass | es_ES |
dc.subject.other | SBA-15 | es_ES |
dc.subject.other | ProOsteon (R) | es_ES |
dc.subject.other | Osteoprogenitor cells differentiation | es_ES |
dc.subject.other | Gentamicin | es_ES |
dc.subject.other | SOL-GEL GLASSES | es_ES |
dc.subject.other | BONE REGENERATION | es_ES |
dc.subject.other | OSTEOBLASTIC DIFFERENTIATION | es_ES |
dc.subject.other | Drug release | es_ES |
dc.subject.other | HYDROXYAPATITE MODEL | es_ES |
dc.subject.other | OSTEOMYELITIS | es_ES |
dc.subject.other | BIOMATERIALS | es_ES |
dc.title | In vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2-CaO-P2O5 scaffold | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.centro | Facultad de Ciencias | es_ES |
dc.identifier.doi | 10.1016/j.micromeso.2016.04.018 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |