The main purpose of the present study was to compare the accuracy of total daily steps thresholds associated to the recommended 60 min per day moderate-to-vigorous physical activity in adolescents. A total of 156 adolescents, 87 males and 69 females, participated in the present study. Objectively-measured moderate-to-vigorous physical activity and steps were assessed by GT3X accelerometers for eight consecutive days. The accuracy of the following total daily steps thresholds was calculated (males/females): 9,930, 11,714, 12,000, 11,000/10,500, 14,000/11,500, 13,000/ 12,000, 8,500/7,500, 11,500/9,000, 10,500/9,500, 12,118, 12,118/12,605, and 10,000. The results of accuracy both uncensored and censored total daily steps cut-off points ranged from low to high. For the uncensored total daily steps, the cut-off 11,000 for males and 10,500 for females showed the best results: Sensitivity = .85; Specificity = .94; Youden’s index = .78; Proportion of agreement = .93, and Kappa coefficient = .67, p < .001. For the censored total daily steps, the 10,000 cut-off score showed the best results: Sensitivity = .77; Specificity = .95; Youden’s index = .72; Proportion of agreement = .93, and Kappa coefficient = .65, p < .001. Among adolescents for accelerometry-measured total daily steps the 11,000 for males and 10,500 for females cut-off points should be used. For population guidelines regarding health-enhancing steps in adolescents, the cut-off point of 10,000 daily steps seems to be the most appropriate.
