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dc.contributor.authorLavado-Benito, Carla
dc.contributor.authorRodríguez-Moreno, Luis Gabriel 
dc.contributor.authorSantamaría-Hernando, Saray
dc.contributor.authorLópez-Solanilla, Emilia
dc.contributor.authorRamos-Rodríguez, Cayo Juan 
dc.date.accessioned2023-10-24T11:19:30Z
dc.date.available2023-10-24T11:19:30Z
dc.date.created2023
dc.date.issued2023
dc.identifier.urihttps://hdl.handle.net/10630/27899
dc.description.abstractChemoreceptors are essential proteins able to detect environmental changes for bacterial adaptation to the environment. The genes encoding these proteins are found individually in the genome or forming clusters with other genes related to chemotaxis. In plant-pathogenic bacteria, about 82 thousand chemosensory sequences have been described (1). In the Pseudomonas syringae complex, one of the most important groups of plant-pathogenic bacteria, four chemotaxisrelated clusters have been described. However, one of these clusters, named cluster II, is absent in some bacteria of this complex infecting woody hosts of the Apocinaceae family. Examples of the absence of this cluster are strains of Pseudomonas savastanoi pv. mandevillae (Psm) and some strains of P. savastanoi pv. nerii (Psn), isolated from dipladenia (Mandevilla spp.) and oleander (Nerium oleander), respectively. Therefore, the aim of this work focuses on the functional characterization of cluster II, not only in bacteria isolated from woody hosts, but also in strains infecting herbaceous plants. First, we constructed knockout mutants of most genes encoded in cluster II, i.e. cheA, cheB, cheD, cheY and two genes coding for chemoreceptors in a woody plant pathogenic strain, both in Psn23 strain and in P. syringae pv. tomato (Pto) DC3000. Motility and virulence assays performed in oleander, dipladenia and tomato plants revealed that cluster II is involved in both phenotypes. In addition, bioinformatic analysis of the ligand-binding domain (LBD) (1) of the two chemoreceptors encoded in cluster II showed that only one of them has an LBD domain. To characterise this chemoreceptor in strain Psn23, capillarity chemotaxis assays are being performed, and its LBD domain has been purified. The purified domain will be used in protein-ligand interaction assays against a collection of plant-derived compounds.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.publisherInternational Society for plant pathologyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBacterias fitopatógenases_ES
dc.subjectPlantas - Enfermedades bacterianases_ES
dc.subject.otherSavastanoies_ES
dc.subject.otherQuimiotaxises_ES
dc.subject.otherClusteres_ES
dc.titleRole of chemotaxis cluster II in pathogenic bacteria of woody and herbaceous plants.es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle12th International Congress of Plant Pathologyes_ES
dc.relation.eventplaceLyones_ES
dc.relation.eventdate20-25 agostoes_ES


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