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dc.contributor.authorMoreno-García, Patricia
dc.contributor.authorÁlvarez-Torres, Daniel
dc.contributor.authorFrétaud, Maxence
dc.contributor.authorMehraz, Manon
dc.contributor.authorLangevin, Christelle
dc.contributor.authorBéjar-Alvarado, Julia 
dc.contributor.authorAlonso-Sánchez, María del Carmen 
dc.date.accessioned2023-09-20T12:58:31Z
dc.date.available2023-09-20T12:58:31Z
dc.date.created2023-09-11
dc.date.issued2023
dc.identifier.urihttps://hdl.handle.net/10630/27615
dc.description.abstractViral nervous necrosis is responsible for important economic losses in aquaculture facilities. The causative agent is the nervous necrosis virus (NNV). Four NNV species have been described, although only RGNNV and SJNNV have been detected in the Mediterranean area. RGNNV- SJNNV reassortants have also been isolated from several species. In order to design strategies to improve fish resistance to NNV, in vivo studies in commercial and model species are required to study the mechanisms underlying fish susceptibility to viral isolates. The aim of this work was to set up zebrafish as model of NNV infection. To fulfil this aim, zebrafish susceptibility to three NNV isolates was determined, and viral replication and innate immune response were characterized. Three days post-fertilisation zebrafish larvae were infected by intracerebral injection with 107 TCID50/mL of SJ93Nag (SJNNV), Dl956 (RGNNV from seabass), and RG/SJ (from seabream). Larvae were daily monitored for 4 days to record clinical signs and mortality. At 1 and 4 days post-infection (dpi), 3 pools of 6 larvae were sampled for viral genome quantification. Innate immune response was also assessed. Transcriptional analyses were completed by in vivo 3D imaging approaches on a zebrafish transgenic line expressing GFP in neutrophils (Tg (mpx:GFP) to monitor neutrophils recruitment in brain. RGNNV was the most virulent isolate compared to SJNNV and RG/SJ. These observations were consistent with viral genome replication, as the highest number of viral genome copies was in RGNNV-infected larvae. The induction of immune-related genes and the recruitment of neutrophils in brain, was also higher in RGNNV-infected larvae. Therefore, further experiments can be designed in this successfully model to better understand the mechanisms underlying NNV virulence in its hosts. Acknowledgments: Projects PID2020-115954RB-100/AEI/10.13039/501100011033 (Spanish Government) and EU H2020 VBN_22_73 (VetBioNet project).es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectRelaciones huésped-viruses_ES
dc.subjectPeces cebraes_ES
dc.subjectPeces - Enfermedades por viruses_ES
dc.subject.otherNodaviruses_ES
dc.subject.otherZebrafishes_ES
dc.subject.otherVirus-host interactiones_ES
dc.titleSetting out zebrafish (Danio rerio) as a model to study nervous necrosis virus-host interaction.es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle21st International Conference on Diseases of Fish and Shellfishes_ES
dc.relation.eventplaceAberdeen, Reino Unidoes_ES
dc.relation.eventdateSeptiembre 2023es_ES


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