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dc.contributor.authorMarrero Capitán, Ana Dácil
dc.contributor.authorPosligua-García, Joel
dc.contributor.authorBernal, Manuel
dc.contributor.authorOrtega Vidal, Juan
dc.contributor.authorSalido, Sofía
dc.contributor.authorAltarejos, Joaquín
dc.contributor.authorRodríguez-Quesada, Ana María 
dc.contributor.authorMartínez-Póveda, Beatriz Amparo 
dc.contributor.authorMedina-Torres, Miguel Ángel 
dc.date.accessioned2023-07-18T09:33:51Z
dc.date.available2023-07-18T09:33:51Z
dc.date.created2023-07-09
dc.date.issued2023
dc.identifier.urihttps://hdl.handle.net/10630/27277
dc.descriptionEs una comunicación a congreso internacional en formato póster.es_ES
dc.description.abstractOur group has recently shown that the antitumor Extra Virgin Olive Oil phenolic compounds (—)oleocanthal and (—)oleacein also behave as antiangiogenic agents. Interestingly, it has been described that phenolic compounds found in the Mediterranean diet affect the autophagy pathway. Based on this background, we studied the modulatory effects of (—)oleocanthal and (—)oleacein on tumor cell autophagy. Methodologically, the tumor cell lines MDAMB231, MCF7 and HT1080 cell lines were used in in vitro cellular and molecular studies of the autophagy flux and key mediators of this process, and High Content Screening (HCS) System using Perkin Elmer Operetta for single-cell analysis was performed in these cells. Interestingly, (—)oleocanthal and (—)oleacein repressed the autophagy flux of MDAMB231 and MCF7 submitted to autophagy inducing conditions (severe starving) at doses in the low micromolar range. In addition, key autophagy mediators, like LC3 or WIPI2 proteins, were dramatically reduced in the same settings, as seen in immunohistochemical studies. Furthermore, preliminary results of HCS in tumor cells revealed depletory effects on autophagy by using specifics dyes for this process at the single-cell level. Altogether, our results point to a drastic inhibitory effect of (—)oleocanthal and (—)oleacein on tumor cell autophagy at low doses.es_ES
dc.description.sponsorship[Grants: PID2022-138181OB-I00, PID2019-105010RB-I00 and RTI2018-098560-BC22 (Spanish Government), UMA18-FEDERJA-220, and PY20_00257 (Andalusian Government and FEDER). Funds from BIO 267 (Andalusian Government) M.B. is supported by “Juan de la Cierva – Incorporation Program” (IJC2018-037657-I), Spanish Ministry of Science and Innovation, Spain.]. Supported with a a help from the «II Plan Propio de Investigación, Transferencia y Divulgación Científica de la UMA», Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAntiangiogénicoses_ES
dc.subjectFenoles - Uso terapéuticoes_ES
dc.subjectAceite de oliva - Uso terapéuticoes_ES
dc.subject.otherExtra virgin olive oiles_ES
dc.subject.otherOleacines_ES
dc.subject.otherOleocanthales_ES
dc.subject.otherAutophagyes_ES
dc.titleThe Extra Virgin Olive Oil phenolic compounds () oleacein and () oleocanthal inhibit tumor cell autophagyes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleThe 47th FEBS Congresses_ES
dc.relation.eventplaceTours (Francia)es_ES
dc.relation.eventdate8-12 de julio de 2023es_ES
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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