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dc.contributor.authorCaballano-Infantes, Estefanía
dc.contributor.authorHo-Plágaro, Ailec
dc.contributor.authorLópez-Gómez, Carlos
dc.contributor.authorMartín-Reyes, Flores
dc.contributor.authorRodríguez-Pacheco, Francisca
dc.contributor.authorTaminiau, Bernard
dc.contributor.authorDaube, Georges
dc.contributor.authorGarrido Sánchez, Lourdes
dc.contributor.authorAlcaín-Martínez, Guillermo
dc.contributor.authorAndrade-Bellido, Raúl Jesús 
dc.contributor.authorGarcía-Cortés, Miren 
dc.contributor.authorLucena-González, María Isabel 
dc.contributor.authorGarcía Fuentes, Eduardo
dc.contributor.authorRodriguez-Diaz, Cristina
dc.date.accessioned2023-06-15T07:36:36Z
dc.date.available2023-06-15T07:36:36Z
dc.date.created2023-06-15
dc.date.issued2023-03-27
dc.identifier.citationCaballano-Infantes E, Ho-Plágaro A, López-Gómez C, Martín-Reyes F, Rodríguez-Pacheco F, Taminiau B, Daube G, Garrido-Sánchez L, Alcaín-Martínez G, Andrade RJ, et al. Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells. Antioxidants. 2023; 12(4):818. https://doi.org/10.3390/antiox12040818es_ES
dc.identifier.urihttps://hdl.handle.net/10630/26958
dc.description.abstractClostridioides difficile infection (CDI) appears to be associated with different liver diseases. C. difficile secretes membrane vesicles (MVs), which may be involved in the development of nonalcoholic fatty liver disease (NALFD) and drug-induced liver injury (DILI). In this study, we investigated the presence of C. difficile-derived MVs in patients with and without CDI, and analyzed their effects on pathways related to NAFLD and DILI in HepG2 cells. Fecal extracellular vesicles from CDI patients showed an increase of Clostridioides MVs. C. difficile-derived MVs that were internalized by HepG2 cells. Toxigenic C. difficile-derived MVs decreased mitochondrial membrane potential and increased intracellular ROS compared to non-toxigenic C. difficile-derived MVs. In addition, toxigenic C. difficile-derived MVs upregulated the expression of genes related to mitochondrial fission (FIS1 and DRP1), antioxidant status (GPX1), apoptosis (CASP3), glycolysis (HK2, PDK1, LDHA and PKM2) and β-oxidation (CPT1A), as well as anti- and pro-inflammatory genes (IL-6 and IL-10). However, non-toxigenic C. difficile-derived MVs did not produce changes in the expression of these genes, except for CPT1A, which was also increased. In conclusion, the metabolic and mitochondrial changes produced by MVs obtained from toxigenic C. difficile present in CDI feces are common pathophysiological features observed in the NAFLD spectrum and DILI.es_ES
dc.description.sponsorshipPartial funding for open access charge: Universidad de Málagaes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCélulas hepáticases_ES
dc.subjectMetabolismoes_ES
dc.subjectVesícula biliares_ES
dc.subjectAparato digestivo-Enfermedadeses_ES
dc.subjectPatógenoses_ES
dc.subjectPatología celulares_ES
dc.subject.otherClostridioides difficilees_ES
dc.subject.otherExtracellular vesicleses_ES
dc.subject.otherMetagenomices_ES
dc.subject.otherLiver diseaseses_ES
dc.subject.otherMetabolic pathwayses_ES
dc.titleMembrane vesicles of toxigenic Clostridioides difficile affect the metabolism of liver hepG2 cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doihttps://doi.org/10.3390/antiox12040818
dc.rights.ccAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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