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dc.contributor.authorCasas Arrojo, Virginia
dc.contributor.authorArrojo-Agudo, María Ángeles 
dc.contributor.authorCárdenas García, Casimiro
dc.contributor.authorCarrillo Fernández, Paloma
dc.contributor.authorPérez Manríquez, Claudia
dc.contributor.authorMartínez-Manzanares, Eduardo 
dc.contributor.authorAbdala-Díaz, Roberto Teófilo 
dc.date.accessioned2023-02-03T08:38:03Z
dc.date.available2023-02-03T08:38:03Z
dc.date.issued2022-11-10
dc.identifier.citationCasas-Arrojo V, Arrojo Agudo MdlÁ, Cárdenas García C, Carrillo P, Pérez Manríquez C, Martínez-Manzanares E, Abdala Díaz RT. Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs. Pharmaceuticals. 2022; 15(11):1379. https://doi.org/10.3390/ph15111379es_ES
dc.identifier.urihttps://hdl.handle.net/10630/25891
dc.description.abstractThe present study was carried out to determine the bioactivity of polysaccharides extracted from Euglena gracilis (EgPs). These were characterized by FT-IR and GC-MS. Cytotoxicity analyses (MTT) were performed on healthy human gingival fibroblast cell lines (HGF-1), obtaining an IC50 of 228.66 µg mL−1, and cell lines with anticancer activity for colon cancer (HCT-116), breast cancer (MCF-7), human leukemia (U-937, HL-60) and lung cancer (NCl-H460), showing that EgPs have anticancer activity, mainly in HTC-116 cells (IC50 = 26.1 µg mL−1). The immunological assay determined the immunomodulatory capacity of polysaccharides for the production of proinflammatory cytokines IL-6 and TNF-α in murine macrophages (RAW 264.7) and TNF-α in human monocytes (THP-1). It was observed that the EgPs had a stimulating capacity in the synthesis of these interleukins. The antioxidant capacity of polysaccharides and their biomass were analyzed using the ABTS method (18.30 ± 0.14% and (5.40 ± 0.56%, respectively, and the DPPH method for biomass (17.79 ± 0.57%). We quantitatively profiled HGF-1 proteins by liquid chromatography–tandem mass spectrometry analysis, coupled with 2-plex tandem mass tag labelling, in normal cells. In total, 1346 proteins were identified and quantified with high confidence, of which five were considered to be overexpressed. The data is available through ProteomeXchange, under identifier PXD029076.es_ES
dc.description.sponsorshipPartial funding for open access charge: Universidad de Málagaes_ES
dc.language.isoenges_ES
dc.publisherIOAP-MDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAlimentos funcionaleses_ES
dc.subject.otherEuglena gracilis; polysaccharideses_ES
dc.subject.otherImmunomodulatory capacityes_ES
dc.subject.otherAntioxidant activityes_ES
dc.subject.otherAnticancer capacityes_ES
dc.subject.otherNutraceuticalses_ES
dc.titleAntioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doihttps://doi.org/10.3390/ph15111379
dc.rights.ccAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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