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dc.contributor.authorArroyo Mateo, Antonio
dc.contributor.authorLeal López, Jesús
dc.contributor.authorRamos-Rodríguez, Cayo Juan 
dc.contributor.authorRamos-Rodríguez, Cayo Juan 
dc.date.accessioned2022-09-06T06:43:23Z
dc.date.available2022-09-06T06:43:23Z
dc.date.created2022-09-05
dc.date.issued2022-07-08
dc.identifier.urihttps://hdl.handle.net/10630/24893
dc.description.abstractThe genome of the olive tree pathogen Pseudomonas savastanoi pv. savastanoi (Psv) NCPPB 3335 encodes a region of about 15 kb named WHOP (from Woody Host and Pseudomonas) which is involved in the catabolism of aromatic compounds and is essential for the virulence of Psv in woody olive plants. This region is shared with other strains of Pseudomonas syringae pathovars infecting woody hosts, but it is absent in strains infecting herbaceous plants. The WHOP region is organized into four operons, antABC (metabolism of anthranilate), catBCA (catabolism of catechol) ipoBCA (oxigenase activity) and dhoAB (degradation of fenolic compounds) and three independently transcribed genes, antR (positive regulator of the antABC operon), PSA3335_3206 (aerotaxis receptor) and whpR (putative AraC family regulator). In this study we identified two domains in WhpR, a DBD (DNA bingding domain), characterised by a classical HTH (Helix-Turn-Helix) motif and an AraC-like bingding domain. BlastP searches showed that no homologs (≥ 60%) of this protein are found outside the P. syringae complex. We also addressed the role of WhpR in virulence by the construction of ΔwhpR mutants in several P. savastanoi strains isolated from olive and oleander (P. savastanoi pv. nerii). Moreover, quantitative real-time PCR (RT-qPCR) analysis of Psv NCPPB 3335 and its ΔwhpR mutant revealed that WhpR is a negative regulator of most of the operons encoded in the WHOP region. Our future aims are to elucidate the mechanism of WhpR-dependent regulation and to determine whether other genes codified outside the WHOP region are also regulated by WhpR.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectPseudomonases_ES
dc.subject.otherPseudomonas savastanoies_ES
dc.subject.otherWHOPes_ES
dc.subject.otheraromatices_ES
dc.subject.otherRegulationes_ES
dc.titleWhpR, an orphan transcriptional regulator of virulence in the pathogen of woody hosts Pseudomonas savastanoi pv. savastanoies_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle14th International Conference on Plant Pathogenic Bacteriaes_ES
dc.relation.eventplaceAssisi, Italyes_ES
dc.relation.eventdateJulio, 2022es_ES


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