The biocontrol rhizobacterium Pseudomonas chlororaphis is one of the bacterial species of the P. fuorescens group where
insecticide ft genes have been found. Fit toxin, supported with other antimicrobial compounds, gives the bacterial the ability
to repel and to fght against eukaryotic organisms, such as nematodes and insect larvae, thus protecting the plant host and
itself. Pseudomonas chlororaphis PCL1606 is an antagonistic rhizobacterium isolated from avocado roots and show efcient biocontrol against fungal soil-borne disease. The main antimicrobial compound produced by P. chlororaphis PCL606
is 2-hexyl-5-propyl resorcinol (HPR), which plays a crucial role in efective biocontrol against fungal pathogens. Further
analysis of the P. chlororaphis PCL1606 genome showed the presence of hydrogen cyanide (HCN), pyrrolnitrin (PRN),
and homologous ft genes. To test the insecticidal activity and to determine the bases for such activity, single and double
mutants on the biosynthetic genes of these four compounds were tested in a Galleria mellonella larval model using inoculation by injection. The results revealed that Fit toxin and HPR in combination are involved in the insecticide phenotype of
P. chlororaphis PCL1606, and additional compounds such as HCN and PRN could be considered supporting compounds
