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dc.contributor.authorGonzález-García, Marta
dc.contributor.authorLópez-González, Manuel Víctor 
dc.contributor.authorCarrillo-Franco, Laura
dc.contributor.authorDíaz-Casares, Amelia 
dc.contributor.authorDawid-Milner, Marc Stefan 
dc.date.accessioned2021-11-11T15:07:11Z
dc.date.available2021-11-11T15:07:11Z
dc.date.created2021
dc.date.issued2021-11
dc.identifier.urihttps://hdl.handle.net/10630/23207
dc.description.abstractThe aim of this study was to characterize the electrophysiological relationships between the CnF and those pontine-medullary neuronal circuits to understand their role in laryngeal control and its effect on vocalization. Objectives: to characterize the role of the CnF in the central mechanisms controlling laryngeal motoneuron activity and its role in vocalization. To achieve this objective is necessary to develop a variation of the classical technique of the “isolated glottis in situ” for the recording of subglottic pressure in rats. Methods: Experimental basic preclinical study in non-inbred male rats. SPF, Sprague-Dawley (250-300 grams) housed under standard conditions have been used. Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2 mg/ kg, i.v., as necessary). A double tracheal (upwards in direction of the glottis for the “glottis isolated in situ” technique, and downwards in the direction of the carina) and esophageal cannulation were performed. Vagus and laryngeal recurrent nerves were isolated and stimulated with bipolar electrodes (Ag/AgCl). Bilateral parietostomy allowed access to the CnF and nA. Electrical stimulation of the CnF using concentric bipolar electrodes was performed (1 ms pulses, 20-40 µA, 100 Hz for 5 s). Subglottic pressure, respiratory flow, pleural pressure, blood pressure, heart rate and unitary neuronal activity were also recorded. Results: Subglottic pressure was recorded in rats with an aneroid transducer (ADInstrument model FE141, ± 0,03 psi) by passing a stream of humidified medical air upwards through the larynx at a constant rate of 50-100 ml/min with a thermal mass digital air flow meter controller (Bronkhorst Hi-Tec F-201CV-AGD-22-V) Conclusions: Our variation of the classical technique for the recording of the “isolated glottis in situ” in rats shows good dynamic responses and can be perfectly used as an index of subglottic pressure and laryngeal activity.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isospaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectNeuronas motorases_ES
dc.subjectLaringees_ES
dc.subjectVocalizaciónes_ES
dc.subject.otherSubglottic Pressurees_ES
dc.subject.otherLaryngeal Motoneuronses_ES
dc.subject.otherCuneiform nucleuses_ES
dc.titleDesign and implementation of a method to study laryngeal resistance during the stimulation of cuneiform nucleus (CnF) in spontaneously breathing anaesthetized ratses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitle19th Meeting of the Spanish Society of Neurosciencees_ES
dc.relation.eventplaceLleidaes_ES
dc.relation.eventdate03-11-2021es_ES


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