β-lactams are the most widely drug prescribed against infections, but they are the most commonly
reported medication allergy too. A correct diagnosis of these allergic reactions is crucial to avoid
rejecting them by other more expensive broad-spectrum antibiotics, with potential risk factors for the
development of multiple drug-resistant bacteria. [1] Skin testing is the most consensual approach to
diagnose β-lactam allergy, but this in vivo test is not risky free and should be performed under strict
hospital surveillance.[2] In vitro testing is not still widely used on account of their low sensitivity. We
report the use of already haptenized fluorescent dendrimers [3] to control the preparation of materials
for in vitro test, and their verification by testing on patient sera samples. This fluorescent dendrimer is
obtained from a dye with two orthogonal functional groups suitable for click chemistry. [4]
Acknowledgments: This work was supported by: MINECO CTQ2016-75870P, Andalusian Regional
Ministry Health (PI-0250-2016); European Regional Development Fund and “Plan Propio Universidad
de Málaga” (UMA-Andalucía-TECH).