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dc.contributor.authorPolonio Escalona, Alvaro Acisclo
dc.contributor.authorSeoane, Pedro
dc.contributor.authorDe-Vicente-Moreno, Antonio 
dc.contributor.authorPérez-García, Alejandro 
dc.date.accessioned2017-07-20T09:29:10Z
dc.date.available2017-07-20T09:29:10Z
dc.date.created2017
dc.date.issued2017-07-20
dc.identifier.urihttp://hdl.handle.net/10630/14279
dc.description.abstractBackgrounds. Cucurbit crops are affected, among other pathogens, by the obligate biotrophic fungus Podosphaera xanthii, the main causal agent of powdery mildew in cucurbits. This fungus develops a specialized structure of parasitism termed haustorium. Haustoria are developed into epidermal cells and are responsible for nutrients uptake and effectors delivery. Objectives. The aim of this study was to obtain the haustorial transcriptome of P. xanthii to complete the panel of effector candidates of this fungal pathogen. Methods. To obtain the haustorial transcriptome, we have developed an effective method for isolation of haustoria without contaminants by flow cytometry. The cDNA library was built using a combination of dT primers and random primers followed by a depletion of ribosomal sequences. Sequencing was carried out by Illumina NextSeq550. Conclusions. After bioinformatic analysis, we were able to identify 25 new effector candidates secreted by the classic pathway (with signal peptide) and 269 new candidates secreted by the non-classic pathway (without signal peptide). Most proteins had no functional annotation. By protein modelling and ligand predictions, we are now being able to assign putative functions to some of these candidates to select those with potential roles in pathogenesis for subsequent functional in vivo analysis by HIGS (host-induced gene silencing). By these approaches, we are starting to shed some light into the molecular mechanisms of pathogenesis in this very important pathogen of cucurbits.es_ES
dc.description.sponsorshipThis work was supported by a grant from the Ministerio de Economía y Competitividad (AGL2013-41938-R), co-financed with FEDER funds (EU). A grant form Universidad de Málaga, Campus de Excelencia Internacional Andalucía Tech, is also acknowledged.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectPodosporaes_ES
dc.subjectOídioses_ES
dc.subject.otherHaustoriumes_ES
dc.subject.otherPodosphaera xanthiies_ES
dc.subject.otherEffector candidateses_ES
dc.subject.otherTranscriptomees_ES
dc.titleDe novo analysis of the haustorial transcriptome of the cucurbit powdery mildew fungus Podosphaera xanthii reveals new candidate secreted effector proteinses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleFEMS2017 7TH CONGRESS OF EUROPEAN MICROBIOLOGISTSes_ES
dc.relation.eventplaceValencia, Españaes_ES
dc.relation.eventdate9 julio 2017es_ES
dc.identifier.orcidhttp://orcid.org/0000-0002-1065-0360es_ES
dc.rights.ccby-nc-nd


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