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dc.contributor.authorRodríguez-Losada, Noela 
dc.contributor.authorWendelbo, Rune
dc.contributor.authorArenas, Ernest
dc.contributor.authorAguirre-Gómez, José Ángel 
dc.date.accessioned2015-09-23T08:12:03Z
dc.date.available2015-09-23T08:12:03Z
dc.date.created2015
dc.date.issued2015-09-23
dc.identifier.urihttp://hdl.handle.net/10630/10291
dc.descriptionEste trabajo presentado inicialmente como contribucion en poster fue seleccionada por el comite organizador por su calidad e invitada para ser presentada como comunicación orales_ES
dc.description.abstractCarbon nanomaterial graphene (G) can form a three-dimensional porous structure with efficient bioconjugation and cell differentiation properties, providing a promising scaffold for neural regeneration. Aims: To study this putative new application of G, we cultured a clonal substantia nigra dopaminergic neuronal progenitor cell line (SN4741) in presence of G as scaffold. Methods: Cells were cultured in DMEM/10% FCS to about 80% confluence and incubated with different concentrations (0.001 to 1 mg/ml) of three chemically different G derivatives (G oxide (GO); partially reduced GO (PRGO) and fully reduced GO (FRGO)) and two different presentation matrixes as powder and films. Cell viability was measured by the MTT assay. To study cellular characterization, morphology and assessment of cell engraftment into G films, we analyzed the immunostaining of the neuronal marker NeuN, the anti-rat Beta-3-tubulin antibody, and the anti-rabbit DCX as immature neuronal marker. Lactate dehydrogenase was measured in the culture supernatant. Results: We found similar increase of survival and metabolism (30-40%) at low concentrations of PRGO and FRGO (0.05-0.01 mg/ml) compared with the higher concentration (1 mg/ml), no changes were seen in the GO group. PRGO or FRGO films showed an increased in the effective anchorage capacity to nest into the G matrix and in the maturation of the dopaminergic SN4741 cells. Conclusions: G scaffolds could offer a powerful platform for neural stem cells, direct cell conversion techniques and neural tissue engineering.es_ES
dc.description.sponsorshipThis work has been supported by the University of Malaga. Campus de Excelencia Internacional Andalucia Tech, and the Norwegian Research Council (grant nº 215086)es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBiomedicinaes_ES
dc.subjectMateriales nanoestructuradoses_ES
dc.subject.othergraphenees_ES
dc.subject.otherdopaminergices_ES
dc.subject.otherSN4741 cellses_ES
dc.subject.otherParkinsones_ES
dc.titlePositive biocompatibility of several graphene derivatives with dopaminergic cells at long term culturees_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitleLatest Advances on Nanomaterials for Biomedical Applicationses_ES
dc.relation.eventplaceBarcelonaes_ES
dc.relation.eventdate21/09/2015es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-3629-4883es_ES
dc.rights.ccby-nc-nd


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