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dc.contributor.authorRodríguez-Negrete, Edgar
dc.contributor.authorLozano-Durán, Rosa
dc.contributor.authorPiedra-Aguilera, Álvaro
dc.contributor.authorCruzado, Lucia
dc.contributor.authorRodríguez-Bejarano, Eduardo 
dc.contributor.authorCastillo-Garriga, Araceli 
dc.date.accessioned2015-07-06T09:21:47Z
dc.date.available2015-07-06T09:21:47Z
dc.date.created2015
dc.date.issued2015-07-06
dc.identifier.urihttp://hdl.handle.net/10630/10025
dc.description.abstractThe apparent simplicity of viruses hides the complexity of their interactions with their hosts. Viruses are masters at circumventing host defenses and manipulating the cellular environment for their own benefit. The replication of the largest known family of single-stranded DNA viruses, Geminiviridae, is impaired by DNA methylation and Arabidopsis mutants affected in cytosine methylation are hypersusceptible to geminivirus infection. This implies that plants might use methylation as a defense against geminiviruses and that the viral genome is a target for plant DNA methyltransferases. We have found a novel counter-defense strategy used by geminiviruses, that reduces the expression of the plant maintenance DNA methyltransferases, MET1 and CMT3, in both, locally and systemically infected tissues. Furthermore, we demonstrated that the virus-mediated repression of these two maintenance DNA methyltransferases is widely spread among different geminivirus species. Additionally, we identified Rep as the geminiviral protein responsible for the repression of MET1 and CMT3, and another viral protein, C4, as an ancillary player in MET1 downregulation. The presence of Rep, suppresses TGS of an Arabidopsis transgene and of host loci whose expression is strongly controlled by CG methylation. Bisulfite sequencing analyses showed that the expression of Rep caused a substantial reduction in the levels of DNA methylation at CG sites. Our findings suggest that Rep, the only viral protein essential for geminiviral replication, displays TGS suppressor activity through a mechanism distinct from the one thus far described for geminiviruses.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectViruses_ES
dc.subjectEpigénesises_ES
dc.subject.otherDNA methylationes_ES
dc.subject.otherGeminiviruses_ES
dc.subject.otherEpigeneticses_ES
dc.subject.otherGene silencinges_ES
dc.titleGeminivirus Rep Protein Interferes with the Plant DNA Methylation Machinery and Suppresses Transcriptional Gene Silencinges_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleThe Wellcome Trust Waddington Symposium. Epigenetics: in dialogue with the genomees_ES
dc.relation.eventplaceEdimburgoes_ES
dc.relation.eventdate1-6-2015es_ES
dc.rights.ccby-nc-nd


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