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Listar por autor "Botella-Mesa, Miguel Ángel"
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Adaptative mechanisms of cellulose synthesis under stress conditions
Pagano Márquez, Raquel; Macho, Alberto P.; Castillo-Garriga, Araceli; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel (2023-07-09)Cellulose is the main structural compound of the plant cell wall and the most abundant biopolymer on Earth (Bar‐On et al 2018). The essential role of cellulose in plant development and defence highlights the importance ... -
Analysis of Arabidopsis SYT1 mutants and chimeras reveal insights about its dynamic and function on ER-PM MCS.
Morello-López, Jorge; Benítez-Fuente, Francisco; Collado, Javier; Fernández-Busnadiego, Rubén; Ruiz-López, Noemí; Botella-Mesa, Miguel Ángel[et al.] (2023)The endoplasmic reticulum (ER) extends throughout the cell and forms Membrane Contact Sites (MCS) with other organelles. MCS are essential for lipid transport, calcium signaling and organelle communication. In plants, ... -
Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacterium tumefaciens
Rosas-Díaz, Tábata Victoria; Cana Quijada, José Francisco; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel; Lozano Durán, Rosa; Bejarano, Eduardo R.[et al.] (Science direct, 2017-02)aken together, our results show that expression of the NahG transgene in Arabidopsis dramatically enhances the efficiency of Agrobacterium-mediated transformation in rosette leaves, enabling the routine use of this technique ... -
Arabidopsis Squalene Epoxidase 3 (SQE3) Complements SQE1 and Is Important for Embryo Development and Bulk Squalene Epoxidase Activity
Laranjeira, Sara; Amorim-Silva, Vitor; Esteban del Valle, Alicia; Arró, Montserrat; Ferrer, Albert; Tavares, Rui M.; Botella-Mesa, Miguel Ángel; Rosado, Abel; Azevedo, Herlânder[et al.] (ScienceDirect, 2015-07-06)The existence of multigenic families in the mevalonate pathway suggests divergent functional roles for pathway components involved in the biosynthesis of plant sterols. Squalene epoxidases (SQEs) are key components of this ... -
Arabidopsis Synaptotagmins 1 and 3 are involved in lipid homeostasis at ER-PM contact sites under cold stress
Amorim-Silva, Vitor; Esteban del Valle, Alicia; Castillo-Garriga, Araceli; Rosado Rey, Abel; Vanneste, Steffen; Perea, Carlos; Salinas, Julio; Botella-Mesa, Miguel Ángel; Ruiz-López, Noemí; Pérez-Sancho, Jessica; Schapire, Arnaldo; Osorio-Algar, Sonia; Willmitzer, Lothar[et al.] (2018-07-12)As sessile organisms, plants must cope with abiotic stress such as soil salinity, drought, and extreme temperatures. This stress signal can activate a phospholipase C (PLC), which hydrolyses PIP2 to generate IP3 and ... -
Autophagy is required for strawberry fruit ripening
Sánchez-Sevilla, José F; Botella-Mesa, Miguel Ángel; Valpuesta-Fernández, Victoriano; Sánchez-Vera, Victoria (Frontiers Media SA, 2021-08-27)Autophagy is a catabolic and recycling pathway that maintains cellular homeostasis under normal growth and stress conditions. Two major types of autophagy, microautophagy and macroautophagy, have been described in plants. ... -
Calling for reinforcements: the role of TTL proteins in the regulación of cell wall biosynthesis
Percio-Vargas, Francisco; Kesten, Christopher; García-Moreno, Álvaro; Amorim-Silva, Vitor; Castillo-Garriga, Araceli; Ruiz-López, Noemí; Sánchez-Rodríguez, Clara; Botella-Mesa, Miguel Ángel[et al.] (2022-09-14)Cellulose is the most abundant organic compound of all biomass on Earth1, with highly relevant roles in plant development and defence. Hence, it is essential to understand the regulation of its biosynthesis to improve the ... -
Characterization of AtDGK2 in relation to Contact Sites
García-Hernández, Selene; Ruiz-López, Noemí; Botella-Mesa, Miguel Ángel (2019-05-17)Contact Sites are conserved cellular regions where two membranes of different organelles are very close but not merged. Contact sites between the endoplasmic reticulum (ER) and the plasma membrane (ER-PM CS) play important ... -
Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
Vallarino Castro, José G.; Merchante-Berg, Catharina; Sánchez-Sevilla, José F; De Luis Balaguer, María Angels; Pott, Delphine; Ariza Fernández, María Teresa; Casañal, Ana; Posé-Padilla, David; Vioque, Amalia; Amaya Saavedra, Iraida; Willmitzer, Lothar; Solano, Roberto; Sozzani, Rosangela; Fernie, Alisdair R; Botella-Mesa, Miguel Ángel; Giovannoni, James J.; Valpuesta-Fernández, Victoriano; Osorio-Algar, Sonia[et al.] (Wiley Online Library, 2019-09-18)FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines ... -
Crucial Roles of Brassinosteroids in Cell Wall Composition and Structure Across Species: New Insights and Biotechnological Applications
Percio-Vargas, Francisco; Rubio-Valverde, Lourdes; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel (Wiley, 2024)Brassinosteroids (BR) are steroidal phytohormones essential for plant growth, development, and stress resistance. They fulfil this role partially by modulating cell wall structure and composition through the control of ... -
Deciphering Strawberry Ripening by Tissue Specific Gene Regulatory Networks
Lucas-Reina, Eva; Trapero-Mozos, Almudena; De Luis Balaguer, María Angels; Martín-Pizarro, Carmen; Ramos-Alvelo, Martín; Sozzani, Rosangela; Botella-Mesa, Miguel Ángel; Valpuesta-Fernández, Victoriano; Posé-Padilla, David[et al.] (2018-07-12)During ripening, fruits undergo a number of metabolic and physiological changes leading to softening and improvement of characters such as flavor and palatability. Insights into transcriptome changes during strawberry fruit ... -
Deciphering the role of plant Synaptotagmins in the tolerance to multiple stresses
Pérez Sancho, Jésica (UMA Editorial, 2017-12-01)Las células de todos los organismos están sujetas a estrés, entendiendo éste como condiciones ambientales desfavorables que limitan su normal desarrollo. A diferencia de otros organismos, las plantas no pueden escapar ... -
Diacylglycerol transport by Arabidopsis Synaptotagmin 1 at ERplasma membrane contact sites under abiotic stress.
Ruiz-López, Noemí; Pérez-Sancho, Jessica; Esteban del Valle, Alicia; García-Hernández, Selene; Huércano Rubens, Carolina; Percio-Vargas, Francisco; Benítez de la Fuente, Francisco; Osorio-Algar, Sonia; Steffen, Vanneste; Lothar, Willmitzer; Napier, Johnathan A.; Perea, Carlos; Salinas, Julio; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel[et al.] (2019-11-13)Bulk lipid transport between membranes within cells involves vesicles, however membrane contact sites have recently been discovered as mediators of non-vesicular lipid transfer. ER-PM contact sites are conserved structures ... -
Ethylene is involved in strawberry fruit ripening in an organ-specific manner.
Merchante, Catharina; Valpuesta, Victoriano; Vallarino, José G.; Osorio-Algar, Sonia; Aragüez, Irene; Villareal, Natalia; Ariza, Maria T.; Martínez, Gustavo A.; Medina-Escobar, Nieves; Civello, Marcos P.; Fernie, Alisdair R.; Botella-Mesa, Miguel Ángel[et al.] (Oxford Academic, 2013-10-05)The fruit of the strawberry F.ananassa has traditionally been classified as non-climacteric because its ripen- ing process is not governed by ethylene. However, previous studies have reported the timely endogenous ... -
FaRIF Transcription Factor Plays a Key Role in the Regulation of Fruit Ripening in the Cultivated Strawberry Fragaria x ananassa
Martín-Pizarro, Carmen; Meco, Victoriano; Vallarino Castro, José G.; Pillet, Jeremy; Casañal, Ana; Merchante-Berg, Catharina; Giovannoni, James J.; Osorio-Algar, Sonia; Botella-Mesa, Miguel Ángel; Posé-Padilla, David; Valpuesta-Fernández, Victoriano[et al.] (2018-07-12)Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, ... -
Farif transcription factor plays a key role in the regulation of fruit ripening in the cultivated strawberry fragaria × ananassa
Martín-Pizarro, Carmen; Meco, Victoriano; Urrutia, María; Vallarino Castro, José G.; Pillet, Jeremy; Casañal, Ana; Osorio-Algar, Sonia; Botella-Mesa, Miguel Ángel; Valpuesta-Fernández, Victoriano[et al.] (2021-01-22)Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, ... -
Functional characterization of Arabidopsis thaliana Synaptotagmin1 domains using Tricalbin3 chimeras in Saccharomyces cerevisiae
Benítez-Fuente, Francisco; Amorim-Silva, Vitor; Collado, Javier; Fernández-Busnadiego, Rubén; Botella-Mesa, Miguel Ángel (2023-06)Synaptotagmin1 (SYT1) is an Arabidopsis thaliana endoplasmic reticulum (ER)-plasma membrane contact site tether involved in biotic and abiotic stress resistance. These resistance roles have been related to SYT1 tethering ... -
Functional characterization of SYNAPTOTAGMIN1 domains in stress resistance
Benítez de la Fuente, Francisco (UMA Editorial, 2024)Synaptotagmina1 (SYT1) es una proteína de Arabidopsis thaliana que se expresa ubicuamente en la planta. Su importancia biotecnológica radica en su esencialidad para la tolerancia a estreses ambientales y su implicación en ... -
Gene expression atlas of fruit ripening and transcriptome assembly from RNA-seq data in octoploid strawberry (Fragaria × ananassa)
Sánchez-Sevilla, José F; Vallarino Castro, José G.; Osorio-Algar, Sonia; Bombarely, Aureliano; Posé-Padilla, David; Merchante-Berg, Catharina; Botella-Mesa, Miguel Ángel; Amaya Saavedra, Iraida; Valpuesta-Fernández, Victoriano[et al.] (Springer Nature, 2017-10-23)RNA-seq has been used to perform global expression analysis of the achene and the receptacle at four stages of fruit ripening, and of the roots and leaves of strawberry (Fragaria × ananassa). About 967 million reads and ... -
Genetic and genome-wide transcriptomic analyses identify co- regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit.
Lima-Silva, Viviana; Rosado, Abel; Amorim-Silva, Vitor; Muñoz-Mérida, Antonio; Pons, Clara; Bombarely, Aureliano; Trelles, Oswaldo; Fernández Muñoz, Rafael; Granell, Antonio; Valpuesta-Fernández, Victoriano; Botella-Mesa, Miguel Ángel[et al.] (BioMed Central, 2012-05-14)L-ascorbic acid (AsA; vitamin C) is essential for all living plants where it functions as the main hydrosoluble antioxidant. It has diverse roles in the regulation of plant cell growth and expansion, photosynthesis, and ...