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ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data
Perkins, James Richard; Dawes, John M; McMahon, Steve B; Bennett, David LH; Orengo, Christine A.; Kohl, Matthias[et al.] (BMC, 2012-07)Background: Measuring gene transcription using real-time reverse transcription polymerase chain reaction (RT-qPCR) technology is a mainstay of molecular biology. Technologies now exist to measure the abundance of many ...