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Listar por autor "Rodríguez-Negrete, Edgar"
Mostrando ítems 1-14 de 14
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A sensitive method to quantify replicative forms of circular DNA viruses
Rodríguez-Negrete, Edgar (2013-12-13)Although real-time PCR diagnostic protocols for detection of geminivirusesare available, none of them are able to discriminate between the two strands generated during an infection: the viral strand (VS) that is encapsidated ... -
Análisis de la infección por geminivirus en plantas con la maquinaria de metilación del DNA alterada.
Piedra-Aguilera, Álvaro; Rodríguez-Negrete, Edgar; Rodríguez-Bejarano, Eduardo; Castillo-Garriga, Araceli (2015-10-26)Los geminivirus son virus de plantas pertenecientes a la familia Geminiviridae. Poseen un genoma compuesto por una o dos moléculas de DNA circular de cadena simple y una cápside compuesta de 2 partes icosaédricas gemelas, ... -
Effector-mediated suppression of plant defense against biotrophs through activation of antagonistic defense against necrotrophs
López-Márquez, Diego; Zumaquero, Adela; Rodríguez-Negrete, Edgar; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario (2015-12-15)Plant hormones are small molecules involved in the regulation of plant growth, development, reproduction and stress responses. Salicylic acid (SA) and jasmonates (JA) are essential for the activation of defence responses ... -
Geminivirus Rep Protein Interferes with the Plant DNA Methylation Machinery and Suppresses Transcriptional Gene Silencing
Rodríguez-Negrete, Edgar; Piedra-Aguilera, Álvaro; Lozano-Durán, Rosa; Cruzado, Lucia; Rodríguez-Bejarano, Eduardo; Castillo-Garriga, Araceli[et al.] (2015-10-20)Viruses are masters at circumventing host defenses and manipulating the cellular environment for their own benefit. The replication of the largest known family of single-stranded DNA viruses, Geminiviridae, is impaired by ... -
Geminivirus Rep Protein Interferes with the Plant DNA Methylation Machinery and Suppresses Transcriptional Gene Silencing
Rodríguez-Negrete, Edgar; Lozano-Durán, Rosa; Piedra-Aguilera, Álvaro; Cruzado, Lucia; Rodríguez-Bejarano, Eduardo; Castillo-Garriga, Araceli[et al.] (2015-07-06)The apparent simplicity of viruses hides the complexity of their interactions with their hosts. Viruses are masters at circumventing host defenses and manipulating the cellular environment for their own benefit. The ... -
miRNA/phasiRNA mediated regulation of plant defense response against P. syringae
López-Márquez, Diego; Del Espino Pérez, Ángel; López-Pagán, Nieves; Rodríguez-Negrete, Edgar; Zumaquero, Adela; Ruiz-Albert, Francisco Javier; Rubio-Somoza, Ignacio; Rodríguez-Bejarano, Eduardo[et al.] (2018-09-10)Gene silencing is a mechanism of regulation of gene expression where the small RNAs (sRNAs) are key components for giving specificity to the system. In plants, two main types of noncoding small RNA molecules have been ... -
PAMP-triggered immunity against Pseudomonas syringae involves microRNA-mediated regulation of several uncharacterized R genes
López-Márquez, Diego; Rodríguez-Negrete, Edgar; López-Pagán, Nieves; Zumaquero, Adela; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario[et al.] (2016-06-27)Two main types of noncoding small RNA molecules have been found in plants: microRNAs (miRNAs) and small interfering RNAs (siRNAs). They differ in their biogenesis and mode of action, but share similar sizes (20-24 nt). ... -
PAMP-Triggered immunity against Pseudomonas syringae involves microRNA-mediated regulation of several uncharacterized R genes
López-Márquez, Diego; Rodríguez-Negrete, Edgar; López-Pagán, Nieves; Zumaquero, Adela; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario[et al.] (2016-10-10)Two main types of noncoding small RNA molecules have been found in plants: microRNAs (miRNAs) and small interfering RNAs (siRNAs). They differ in their biogenesis and mode of action, but share similar sizes (20-24 nt). ... -
Papel del silenciamiento genético en la regulación de genes R durante la interacción con Pseudomonas syringae
López-Márquez, Diego; Rodríguez-Negrete, Edgar; López-Pagán, Nieves; Zumaquero, Adela; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario[et al.] (2016-10-11)El silenciamiento génico es un mecanismo de regulación de la expresión génica mediado por pequeños RNAs. En plantas, los precursores de estos pequeños RNAs, son procesados por proteinas Dicer-Like (RNAse III). Existen dos ... -
R gene regulation mediated by miRNA/phasiRNA during plant defense response against P. syringae
López-Márquez, Diego; Del Espino Pérez, Ángel; López-Pagán, Nieves; Rodríguez-Negrete, Edgar; Ruiz-Albert, Francisco Javier; Rubio-Somoza, Ignacio; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario[et al.] (2019-07-26)In plants, two main types of noncoding small RNA molecules have been found: microRNAs (miRNAs) and small interfering RNAs (siRNAs), differing these in their biogenesis and mode of action, but sharing similar sizes (20-24 ... -
Regulación de genes de resistencia de la familia TIR-NBS-LRR mediada por miRNA/phasiRNA durante la interacción con P. syringae
Rodríguez-Negrete, Edgar; Zumaquero, Adela; Ruiz-Albert, Francisco Javier; Rubio-Somoza, Ignacio; Rodríguez-Bejarano, Eduardo; Beuzón-López, Carmen del Rosario; López-Márquez, Diego; López-Pagán, Nieves; Del Espino Pérez, Ángel[et al.] (2018-10-24)Durante un estrés biótico, las plantas modulan la expresión de una batería de genes involucrados en la respuesta de defensa, proceso donde recientemente se ha determinado el papel esencial que desempeña el silenciamiento ... -
Study of the functional domains of the PTGS suppressor V2 from geminivirus Beet curly top virus (BCTV)
Luna, Ana P.; Rodríguez-Negrete, Edgar; Morilla, Gabriel; Castillo-Garriga, Araceli; Rodríguez-Bejarano, Eduardo (2019-07-25)Geminiviruses constitute a group of plant viruses that infect vegetable crops all over the world. Among the Geminiviridae family, the genera Mastrevirus, Begomovirus and Curtovirus are the most abundant. Suppression of ... -
Study of the functional domains of V2 from Beet curly top virus (BCTV)
Luna, Ana P.; Rodríguez-Negrete, Edgar; Morilla, Gabriel; Castillo-Garriga, Araceli; Rodríguez-Bejarano, Eduardo (2018-10-29)Geminiviruses constitute a group of plant viruses that infect vegetable crops all over the world. Among the Geminiviridae family, the genera Mastrevirus, Begomovirus and Curtovirus are the most abundant. Suppression of ... -
Study of the PTGS suppressor activity of V2 protein from geminivirus Beet curly top virus
Pérez-Luna, Ana Isabel; Rodríguez-Negrete, Edgar; Castillo, D.; Morilla, Gabriel; Wang, Liping; Lozano-Durán, Rosa; Rodríguez-Bejarano, Eduardo[et al.] (2017-06-26)Suppression of gene silencing is a key mechanism for the success of viral infection in plants. DNA viruses from the Geminiviridae family encode several proteins that suppress post- and transcriptional gene silencing ...