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Generation and use of site-directed chromosomal cyaA’ translational fusions in Salmonella enterica.
Ramos Morales, Francisco; Cardenal Muñoz, Elena; Cordero Alba, Mar; Baisón Olmo, Fernando (Springer, 2015)CyaA from Bordetella pertussis is a calmodulin-dependent adenylate cyclase. Fusions to the catalytic domain of CyaA (CyaA’) are useful tools to detect translocation of type III secretion system effectors from Gram-negative ...