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Mostrando ítems 1-17 de 17
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Antisense down-regulation of the strawberry β-galactosidase gene FaβGal4 increases cell wall galactose levels and reduces fruit softening
Paniagua Correas, Candelas; Blanco-Portales, Rosario; Barceló-Muñoz, Marta; García-Gago, Juan Antonio; Waldron, Keith W.; Quesada-Felice, Miguel Ángel; Muñoz-Blanco, Juan; Mercado, José A.[et al.] (Oxford University Press, 2015-11-19)Este estudio investigó el papel del gen FaβGal4 en el reblandecimiento de la fresa durante la maduración. Se identificó que FaβGal4 está implicado en la solubilización de pectinas a través de la liberación de galactosa, ... -
Caracterización funcional de genes reguladores de la maduración en fresa y aplicación del sistema CRISPR/Cas9 para alargar la vida postcosecha y mejorar la calidad organoléptica del fruto.
Sánchez-Raya, Cristina (UMA Editorial, 2024)Este trabajo de investigación ha sido realizado por Cristina Sánchez-Raya en el grupo de Fisiología Vegetal del Departamento de Botánica y Fisiología Vegetal de la Universidad de Málaga, España, bajo la codirección de la ... -
Cell wall disassembly is delayed by rhamnogalacturonate lyase gene silencing: potential role in fruit firmness
Pose-Albacete, Sara; Ric-Varas, P.; Schückel, J.; Molina-Hidalgo, F.J.; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Knox, John Paul; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2019-07-23)Strawberry fruits greatly reduce their quality due to softening during ripening with economically important losses. Texture changes of fleshy fruits during ripening are mainly due to middle lamellae dissolution, cell-to-cell ... -
CRISPR/Cas9 editing of the polygalacturonase FaPG1 gene improves strawberry fruit firmness
López-Casado, Gloria; Sánchez-Raya, Cristina; Ric-Varas, Pablo; Paniagua Correas, Candelas; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Posé-Albacete, Sara; Matas, Antonio J.; Mercado-Carmona, José Ángel[et al.] (Oxford Academy, 2023-02-01)La firmeza es uno de los rasgos de calidad más importantes en las fresas. La vida útil postcosecha de esta fruta está altamente limitada por la pérdida de firmeza, donde la descomposición de la pared celular juega un papel ... -
Down-regulation of a pectin acetylesterase gene modifies strawberry fruit cell wall pectin stracture and increases fruit firmness
Matas-Arroyo, Antonio Javier; Rodríquez, Virginia; Sánchez, Luis; Paniagua Correas, Candelas; Pose-Albacete, Sara; García-Gago, Juan Antonio; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Mercado-Carmona, José Ángel[et al.] (2015-06-29)Antisense-mediated down-regulation of several fruit-specific genes has previously demonstrated how the cell wall disassembly in strawberry fruit is mediated by a series of enzymes that act sequentially (Posé et al. 2011). ... -
Downregulation of NAC transcription factors modifies cell wall composition and increases strawberry fruit firmness
Ric-Varas, Pablo; Pose-Albacete, Sara; López-Casado, Gloria; Marcus, S.E.; Knox, John Paul; Blanco-Portales, Rosario; Moyano, E.; Muñoz-Blanco, Juan; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (2019-07-23)The strawberry is a soft fruit with a very short post-harvest shelf life. The changes in texture during fruit ripening are mainly due to the dissolution of the middle lamellae, reducing cell-to-cell adhesion, and the ... -
Efecto del silenciamiento de genes que codifican poligalacturonasas sobre el reblandecimiento del fruto de fresa asociado a la maduración
Paniagua Correas, Candelas; García-Gago, Juan Antonio; Matas-Arroyo, Antonio Javier; Barceló-Muñoz, Marta; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Pliego-Alfaro, Fernando; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2015-10-06)La degradación de las pectinas de la pared celular mediada por poligalacturonasas juega un papel clave en el reblandecimiento de la fresa. Así, el silenciamiento del gen FaPG1 incrementa la firmeza del fruto maduro y alarga ... -
Elucidating the role of polygalacturonase genes in strawberry fruit softening
Paniagua, Candelas; Ric-Varas, Pablo; García-Gago, Juan Antonio; López Casado, Gloria María; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Schückel, Julia; Knox, John Paul; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Posé-Albacete, Sara; Mercado-Carmona, José Ángel[et al.] (Oxford Academy, 2020-12-31)En dicho artículo se investigó el papel de los genes de poligalacturonasa (FaPG1 y FaPG2) en el reblandecimiento de la fresa. El silenciamiento simultáneo de ambos genes no aumentó aún más la firmeza. A nivel genético, ... -
Evaluation of the effect of drought stress on fruit quality of transgenic strawberry plants with cell wall encoding genes down-regulated.
Rodríguez-Hiraldo, Claudia; Aguado, Sara; Posé-Albacete, Sara; Paniagua, Candelas; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (2024)Strawberry is an important crop in our country. One of the main challenges of this crop is to produce high-quality fruits with a prolonged post-harvest shelf life. Strawberries are particularly susceptible to water stress ... -
Generation and analysis of ESTs from strawberry (Fragaria xananassa) fruits and evaluation of their utility in genetic and molecular studies
Bombarely, Aureliano; Merchante-Berg, Catharina; Csukasi, Fabiana; Cruz-Rus, Eduardo; Caballero, José L.; Medina-Escobar, Nieves; Blanco-Portales, Rosario; Botella, Miguel A.; Muñoz-Blanco, Juan; Sánchez-Sevilla, José F; Valpuesta-Fernández, Victoriano[et al.] (BMC, 2010-09-17)Cultivated strawberry is a octoploid species whose fruit is highly appreciated due to its organoleptic properties and health benefits. However, information about the role played by different hormones on these processes ... -
Insights into the effects of polygalacturonase FaPG1 gene silencing on pectin matrix disassembly, enhanced tissue integrity, and firmness in ripe strawberry fruits,
Posé-Albacete, Sara; Paniagua, Candelas; Cifuentes-Rueda, Manuel; Blanco-Portales, Rosario; Mercado-Carmona, José Ángel (Oxford Academy, 2013-07-19)Este estudio examina el impacto de la reducción del gen FaPG1 en fresas, que se ha relacionado con la disminución del reblandecimiento de la fruta y la prolongación de la vida útil postcosecha. Se encontró que las fresas ... -
Isolation and transfection of strawverry protoplasts for gene editing
Sánchez-Raya, Cristina; López-Casado, Gloria; Blanco-Portales, Rosario; Pose-Albacete, Sara; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (2021)Strawberry is the most economically important soft fruit. The improvement of the organoleptic qualities of ripe fruit and the postharvest shelf life are main objectives of strawberry breeding programs. Fruit softening is ... -
Optimización de la transfección de protoplastos para la edición génica en fresa
Sánchez-Raya, Cristina; López-Casado, Gloria; Blanco-Portales, Rosario; Pose-Albacete, Sara; Pliego-Alfaro, Fernando; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel; Pliego-Alfaro, Fernando[et al.] (2021)La fresa es un fruto blando de gran importancia económica, particularmente en Andalucía. La mejora de las cualidades organolépticas del fruto y la disminución del reblandecimiento para alargar la vida postcosecha del fruto, ... -
Polygalacturonase gene FaPG1 downregulation is related to increased strawberry fruit resistance to fungal decay
Paniagua Correas, Candelas; Sánchez-Raya, Cristina; Blanco-Portales, Rosario; Mercado-Carmona, José Ángel; Palomo-Ríos, Elena; Pose-Albacete, Sara[et al.] (2022-06-16)Plant health is a major target in breading programs because crops are under constant biotic stress, and climate change is exacerbating pests and disease negative impacts in agriculture. Obtaining crop varieties armed with ... -
Rhamnogalacturonase lyase gene downregulation in strawberry and its potential on mechanical fruit properties
Ric-Varas, Pablo; Pose-Albacete, Sara; Barceló-Muñoz, Marta; Knox, John Paul; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2017-07-04)Strawberry softening is one of the main factors that reduces fruit quality and leads to economically important losses. Textural changes during fruit ripening are mainly due to the dissolution of middle lamellae, a reduction ... -
Strawberry fruit quality and production under drought stress conditions.
Aguado, Sara; Rodríguez-Hiraldo, Claudia; Posé-Albacete, Sara; Paniagua, Candelas; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Quesada-Felice, Miguel Ángel; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (2024)The increase in temperature and the decrease in water resources predicted by climate change models will directly influence the productivity, ripening rate, and organoleptic quality of fruit crops. Strawberry is an important ... -
Suppressing the rhamnogalacturonan lyase gene FaRGLyase1 preserves RGI pectin degradation and enhances strawberry fruit firmness,
Ric-Varas, Pablo; Paniagua, Candelas; López Casado, Gloria María; Molina-Hidalgo, F.J.; Schückel, Julia; Knox, Paul; Blanco-Portales, Rosario; Moyano, Enriqueta; Muñoz-Blanco, Juan; Posé-Albacete, Sara; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (Elsevier, 2023-12-23)Este estudio investigó el papel de las rhamnogalacturonano liasas(RGLiasas) en el reblandecimiento de la fresa. Se generaron plantas de fresa transgénicas con el gen FaRGLyase1 silenciado, y sus frutos resultaron ser ...