GENERAL INFORMATION 
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1. Dataset title:
Proteomic data of murine hearts with conditional deletion of the gene Wt1

2. Authors:
Díaz del Moral, Sandra; Muñoz-Chápuli, Ramón; Carmona, Rita.
3. Author contact information: 
Ramón Muñoz-Chápuli chapuli@uma.es
Rita Carmona rita@uma.es

METHODOLOGICAL INFORMATION 
1. Description of the methods for collection/generation of data: 
Proteomic analysis was performed on ventricular samples of four control mice and three mice 
with conditional deletion of WT1 in cardiomyocytes. Hearts of mice treated chronically with 
doxorubicin were also analyzed (two controls and three mutants). The protein concentrations 
of ventricular samples were determined by the bicinchoninic acid protein assay and adjusted to 
the same concentration. For quantitative proteomics, each digested protein sample was 
labelled with an appropriate TMT2plex Isobaric Label Reagent (Thermo Fisher Scientific) 
according to the protocol supplied by the manufacturer. Briefly, the wild type and knockout 
peptide samples were labelled with reagents TMT-126 and TMT-127, respectively. To quench 
the TMT reaction, 8 ?L of 5% hydroxylamine was added to each sample and incubated for 15 
min at room temperature. All labelled peptides were combined in equal amounts and dried 
using a speed vacuum system. The peptides were dissolved in 0.1% formic acid and analyzed 
using a Q-Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher 
Scientific) connected to an Easy-nLC 1200 UHPLC (Thermo Fisher Scientific). The peptides and 
proteins were identified and quantified using Proteome DiscovererTM 2.4 (Thermo Fisher 
Scientific) platform and SwissProt Mus musculus protein database version 2017.10.25 (25,097 
sequences). 
More details available in the paper: 
Cardiomyocyte-specific Wt1 might be involved in cardiac metabolism and response to damage 
(International Journal of Molecular Sciences, pending of acceptance). 
2. Data processing methods
The peptides and proteins were identified and quantified using Proteome DiscovererTM 2.4 
(Thermo Fisher Scientific) platform and SwissProt Mus musculus protein database version 
2017.10.25 (25,097 sequences). To calculate the ratio of TMT-labeled proteins between wild 
type and knockout, abundances were based on precursor intensities. Normalization was 
performed based on total peptide amount, and samples were scaled on all average (for every 
protein and peptide the average of all samples is 100). Protein ratios were directly calculated 
from the grouped protein abundances. Abundance ratio p-values were calculated by ANOVA 
based on the abundance of individual proteins.
3. Software or instruments needed to interpret the data:
Excel 2007 

4. Standards and calibration information, if appropriate: 
-

5. Environmental or experimental conditions:
-

FILE OVERVIEW 
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Dataset Proteomic Data KO-WT Untreated:  Comparison between mutant and control mice
Dataset Proteomic Data KO-WT DOXO:  Comparison between mutant and control mice treated 
with doxorubicin


MORE INFORMATION
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